T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice

T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice

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Introduction: Sambucus is a genus of the Adoxaceae family and has been widely used as a food and medicinal source.In this study, we aimed to evaluate the effects of S.javanica extracts toward T cells regulation in the 7,12-dimethylbenzene (a) anthracene (DMBA)-exposed mice.

Methods: Thirty mice were used in this experiment which divided into six treatment groups and five times repetition.Three-month-old BALB/c mice were administrated with 2.8 mg.

kg-1 BW of DMBA as carcinogen agent for ten times within four weeks, then continuously treated with 400 and 800 mg.kg-1 BW of S.javanica berries leaves extracts daily for two weeks.

Treatment groups in this study ethical nutrients mega magnesium powder citrus 450g were vehicle as negative control, DMBA 2.8 mg.kg-1 BW as positive control; BD1 group, DMBA 2.

8 mg.kg-1 + berries extracts 400 mg.kg-1 BW; BD2 group, DMBA 2.

8 mg.kg-1 + berries extracts 800 mg.kg-1 BW; LD1 group, DMBA 2.

8 mg.kg-1 + leaves extract 400 mg.kg1 BW; and LD2 group, DMBA 2.

8 mg.kg-1 + leaves extract 800 mg.kg-1 BW.

The relative number of CD4+CD25+CD62L+ cells and CD4+CD62L+ cells were measured and analyzed by using flow cytometer.Results: DMBA induction decreased the relative number of CD4+CD25+CD62L+ cells while the leaves here extract of S.javanica significantly increased their expression.

Furthermore, DMBA promoted the relative number of CD4+CD62L+ cells expression while leaves and berries extracts of S.javanica sharply suppressed its expression.Conclusion: The results suggest that, S.

javanica extracts may exert their medicinal properties by modulating T cells regulation in DMBA-induced mice.